Table 2. Induction of DNA sequence alterations at targeted loci.

Targeted locus	Amount TALEN RNA injected	# embryos	# (%) embryos with mutations
golden [exon 2]	Uninjected	7	0 (0%)
	100 pg	42	42 (100%)
ryr3 [exon 5]	Uninjected	16	0 (0%)
	100 pg	47	46 (97.9%)
tbx6 [exon 3]	Uninjected	25	0 (0%)
	100 pg	54	54 (100%)
ryr1a [exon 6]	Uninjected	24	0 (0%)
	100 pg	24	24 (100%)

One cell stage embryos were injected with TALEN mRNAs as indicated. gDNA from individual 1–2 dpf embryos was analyzed by HRMA for sequence alterations at targeted loci.