Figure 3. The C-terminus of Nora virus VP1 is essential for RNAi suppressor activity.

(A) Schematic presentation of full-length (FL) and N- and C-terminal deletion mutants (ΔN and ΔC) of VP1. (B) Western blot analysis of VP1 expression constructs. V5 epitope tagged expression constructs were transfected into Drosophila S2 cells and expression of VP1^FL and the deletion mutants was analyzed by Western blot using a V5 antibody (αV5). (C) RNAi reporter assay in S2 cells. The experiment was performed as described in the legend to Figure 2D, using plasmids encoding either CrPV 1A, VP1^FL or the VP1 deletion mutants. Bars represent averages and standard deviations of three independent samples. The graph is representative for two independent experiments.