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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Hepatology. 2012 Oct 14;56(5):1602–1610. doi: 10.1002/hep.25821

Fig. 1. Analysis of B-cells by flow cytometry.

Fig. 1

(A) B-cells were identified using time, single cell and lymphocyte and CD19+ gates. Dead cells, T-cells, NK cells and macrophages were excluded.

(B) CD19+ B-cells were grouped into CD10− mature and CD10+ immature subsets. Mature B-cells were classified into plasmablasts (PB), activated (Act), tissue-like memory (TLM), naïve (N) and resting memory (RM) B-cells based on CD21 and CD27 expression. Immature transitional (CD10+CD27−) B-cells were classified as CD21− T1 or CD21+ T2 cells. Gates were based on fluorescence-minus-one controls.