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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Hypertension. 2012 Jul 9;60(3):697–704. doi: 10.1161/HYPERTENSIONAHA.112.198242

Figure 1. Deletion of USF-15, Sp1-520 and PRA/B-563 inhibits ERα promoter activity.

Figure 1

A 2035 bp fragment of ovine ERα promoter region spanning −2000 to +35 bp relative to the transcription start site was amplified by PCR and inserted into pGL3 to yield the full-length promoter–reporter plasmid (WT-ERα). Three site-specific deletion mutations at USF-15 (ΔUSF-ERα), Sp1-520 (ΔSp1-ERα), and PRA/B-563 (ΔPRA/B-ERα) were constructed. Constructs were then transiently transfected to uterine arterial smooth muscle cells. Firefly and Renilla reniformis luciferase activities were measured after 48 hours in a luminometer using a dual-luciferase reporter assay system. Data are means ± SEM, * P < 0.05, versus WT-ERα. n = 6–10