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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Hypertension. 2012 Jul 9;60(3):697–704. doi: 10.1161/HYPERTENSIONAHA.112.198242

Figure 3. SP1 and USF binding to unmethylated and methylated binding elements at ERα promoter.

Figure 3

Nuclear extracts (NE) from uterine arteries were incubated with double-stranded oligonucleotide probes containing unmethylated (UM) or methylated (M) Sp1-520 binding site (A) or USF-15 binding site (B), in the absence or presence of antibodies (Ab) against Sp1, USF1, or USF2. Cold competition (CC) was performed with unlabeled competitor oligonucleotide at a 200-fold molar excess. Free oligo, no nuclear extracts were added. S, shift; SS, supershift.