Table 3.

Pattern of expression of transient receptor potential vanilloid channels in vascular endothelial cells as detected by reverse transcription/polymerase chain reaction, immunostaining for cultured cells, immunohistochemistry, Western blotting, and in situ hybridization

	TRPV1	TRPV2	TRPV3	TRPV4	TRPV5	TRPV6
PCa/PNa and conductance (pS)[202]	10, 35-80	1-3, NM	12, 172	6, 90	> 100, 75	> 100, 40-70
Human coronary artery ECs[300]				(+RT-PCR, WB, IHC)
Human pulmonary artery ECs[415]	+(RT-PCR)	+(RT-PCR)		+(RT-PCR)
Human pulmonary microvascular ECs	+(RT-PCR)
Human cerebral microvascular ECs[272]	+(RT-PCR, IC)
Human cerebral arterioles ECs[305]			+(RT-PCR, IHC)
Human dermal microvascular ECs[319]				+(RT-PCR, WB)
Breast cancer derived microvascular ECs[319]				+(RT-PCR, WB)
Human umbilical vein ECs[227,277,296,301]	+(RT-PCR)			+(WB, IHC)
Mouse aortic ECs[280,285,299,306]	+(WB)			+(RT-PCR, WB, NM, IHC)
Mouse pulmonary artery ECs[313]				+(WB, IHC)
Mouse mesenteric artery ECs[27,280,302]	+(RT-PCR, WB, IC)			+(RT-PCR, IC)
Mouse cerebral microvascular ECs		+(RT-PCR)		+(RT-PCR, WB)		+(RT-PCR)
Mouse dermal microvascular ECs[307]				+(RT-PCR, WB)
Mouse carotid artery ECs[290,296]				+(IHC)
Rat mesenteric artery ECs[226,275,302]	+(WB)			+(WB, IC, IHC)
Rat femoral artery ECs[312]				+(RT-PCR, IC)
Rat pulmonary artery ECs[303]				+(WB, IHC)
Rat renal artery ECs[303]				+(IHC)
Rat cardiac microvascular ECs[303,316]				+(IHC)
Rat cerebral artery ECs[287,297,311]		+(RT-PCR)	+(RT-PCR, IC)	+(RT-PCR, IC)
Rat carotid artery ECs[291]				+(RT-PCR)
Porcine aortic ECs[312,317]				+(RT-PCR, IC, WB)
Porcine coronary artery ECs[273]	+(IHC)
Bovine aortic ECs[271]	+(WB)
Bovine adrenal capillary ECs[292]				+(RT-PCR, WB)
Zebrafish ventricular ECs				+(ISH)

RT-PCR: Reverse transcription/polymerase chain reaction; WB:Western blotting; IC: Immunostaining for cultured cells; IHC: Immunohistochemistry; ISH: In situ hybridization; NM: Not measured; ECs: Endothelial cells.