Table 2.
List of relevant polyamine biosynthetic genes in A. thaliana analyzed by transcript accumulation.
| Gene | Annotation | Primer sequence (5′ → 3′) | |
|---|---|---|---|
| S-adenosyl-l-methionine decarboxylase (SAMDC) | SAMCD1 | At3g02470 | for TTGAAGTGTGAATGGAGCAG |
| rev GATTCCCTCGTCCTTCTCG | |||
| SAMDC2 | At5g15950 | for GGAAGGTATTGTGGTTCTCC | |
| rev CTATTCCTTCTCGTCCTCGC | |||
| SAMDC3 | At3g25570 | for GGTATTGGCGTCTGATTGTG | |
| rev CCAAGTCCAAGCTCCTCTAG | |||
| SAMDC4 | At5g18930 | for GAGCCGTCTTATGGATGAG | |
| rev CTATTTCCGACGAGGCGT | |||
| Spermidine synthase (SPDS) | SPDS1 | At1g23820 | for CTCGGAGATATTCACCACAG |
| rev CTGATCTCCGTTCTCCGTCT | |||
| SPDS2 | At1g70310 | for ACTGATTTGCCCGTGAAGAG | |
| rev GTTCTCTGTTTCCATGGCGC | |||
| Spermine synthase (SPMS) | SPMS | At5g53120 | for TTCTTCAGATCCCGTAGGTC |
| rev CTCTAGCCAGTGTCTCGAA | |||
| Thermospermine synthase (TSPMS/ACL5) | TSPMS/ACL5 | At5g19530 | for GCTCCTTCTTTCGTCTCTG |
| rev CAGTCTCCTTCTCTAGCGC | |||
| Polyamine oxidase (PAO) | PAO1 | At5g13700 | for ACCCGGGCTCTAACATTC |
| rev GATTGAGCTTCAACGCGC | |||
| PAO2 | At2g43020 | for TTCTGGAGCGGTATGGTG | |
| rev GAAGAGGTACAGAGGCAGG | |||
| PAO3 | At3g59050 | for GAGACTGAGAGTGCCATTG | |
| rev GAATAAGCACCGTGCACTG | |||
| PAO4 | At1g65840 | for CAGGGAATCTAGCACAAGAC | |
| rev CACTAGATATTGAGCCGGGT | |||
| PAO5 | At4g29720 | for GAAGAACCGCGACCATTAC | |
| rev GTTTCTCAAGCTCGAGAGC | |||
| Spermidine disinapoyl transferase (SDT) | SDT | At2g23510 | for TATTGGGATTTTCGGATCG |
| rev CCATATCCGATTCCAGCCTAGA | |||