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. 2012 Jul 30;109(33):13225-13230. doi: 10.1073/pnas.1202509109

Fig. 1.

Fig. 1.

Flow cytometry and fluorescence microscopy of cell wall-deficient or wild-type C. reinhardtii treated with Fl, noncovalent mixture Fl+r8, or Fl-r8 conjugate. Graphs of the mean fluorescence from flow cytometry of (A) cell wall-deficient cc-4350 or (D) wild-type C. reinhardtii. Inset in each graph is a representative histogram from a single condition in the flow cytometry data where blue is the noncovalent Fl+r8 control and red is Fl-r8 conjugate, both at 25 μM. Confocal Z-layers of (B) cell wall-deficient and (E) wild-type cells treated with the noncovalent Fl+r8 control have no apparent internalization. Confocal Z-layers of (C) cell wall-deficient and (F) wild-type cells treated with Fl-r8 conjugate show internalization and not surface staining. The letter over the bar graph indicates the treatment conditions used for the corresponding image. (C, F) Scale bar equals 5 μm.