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Flow cytometry data of C. reinhardtii treated at 4 °C, in the dark and after acid-induced deflagellation. In all graphs the mean fluorescence is plotted, error is SD. (A) Cell wall-deficient cc-4350 and (B) wild-type treated at room temperature vs. 4 °C. (C) Treatment of wild-type in the dark vs. ambient light. (D) Wild-type cells were deflagellated and one sample was immediately treated while a second sample was allowed to recover for 20 min and then treated.
