Fig. 2.

Expanded CAG RNAs interfered with the formation of rRNA transcription preinitiation complex and promoted hypermethylation of rRNA promoter. (A and B) Chromatin immunoprecipitation of the largest subunit of RNA polymerase I (RPA194) and rRNA promoter (A) and upstream binding factor (UBF) and upstream control element (UCE) (B) in CAG RNA-expressing cells. “+” indicates that antibody was present in the immunoprecipitation reactions and “−” indicates that antibody was not included in the reactions. (C) The effect of DNA methyltransferase inhibitor 5-azacytidine on expanded CAG RNA-induced UCE CpG DNA hypermethylation. Real-time PCR analysis of UCE DNA methylation status is shown. Error bars represent ±SD. All experiments were repeated three times.