Fig. 4.

Activation of the BR pathway counteracts the activity of gai-1. (A) Scheme depicting the experimental design. Two-day-old dark-grown, wild-type Col-0 and HS::gai-1 seedlings were transferred to plates containing 3 μM BRZ for an additional day. Half the seedlings were mock-treated with 3 μM BRZ (blue), and half were treated with 1 μM EBR + 3 μM BRZ (red) starting 1 h before heat shock (HS) for 1 h at 37 °C. Samples were harvested 4 h after the beginning of the heat shock. (B) Changes in expression induced by HS::gai-1 in selected target genes expressed relative to EF1α. Three biological repeats were performed; error bars represent SEM. *P < 0.01 (Student’s t test).