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. 2012 Jun 25;109(33):13237–13242. doi: 10.1073/pnas.1200105109

Fig. 5.

Fig. 5.

(A) Homology model of the ERBB2/ERBB3 heterodimer places the A30 interface on the side of the ERBB2/ERBB3 heterodimer, distant from the canonical dimerization interface and on the edge of a unique positively charged surface patch within ERBB3. (B) Cartoon of the heterodimer in the plasma membrane (PM) with indicated A30-binding site and charge complimentary interface on ERBB3 in dark blue. Cytoplasmic components are not drawn to scale. The structure-derived cartoon outline for the Top view highlights the interlocking canonical dimer interface, the charge complimentary interface on ERBB3 (blue), and the binding sites for A30 and NRG. (C) Ligand-enhanced coimmunoprecipitation of overexpressed V5 and Dendra tagged ERBB2 in the presence of low endogenous ERBB3 in MCF7 cells. The V5 epitope tag was immunoprecipitated. Ligand or A30 were added as indicated. (D) Proposed modes of receptor interactions and impact of A30 at low endogenous receptor levels compared with ERBB3 overexpression. Arrows indicate the directionality of tyrosine phosphorylation with arrow thickness indicating relative contributions.