Table 1.
Forward and reverse primer sequences used in real-time PCR, annealing temperatures, resulting amplicon lengths and PCR efficiencies.
| Unigenes | qPCR Forward primer sequences (5' to 3') | qPCR Reverse primer sequences (5' to 3') | Annealing T (°C) | Amplicon lenght (pb) | Efficiency |
|---|---|---|---|---|---|
| SlitOR7 | CCTTCCTATCGATGGCTCTG | CCCAGGTACCACTTGCAGTT | 60 | 115 | 2.1 |
| SlitOR10 | TTGCACTTTATGGGCAATGA | GAAGAGGAAAAGCGCTGATG | 62 | 188 | 1.9 |
| SlitOR12 | TTGGCCTTGGGTGTATCTTC | AAACGGCCACAAGTCTCATC | 62 | 171 | 2.1 |
| SlitOR19 | AAACGTGACTCCGTGAGCTT | CCGCCATCAACGTATTTTCT | 62 | 148 | 2.3 |
| SlitOR24 | CGCATCCGTTTATCGACTTT | CAAACCAGACCACAAGAGCA | 60 | 116 | 2.1 |
| SlitOR32 | GGTACTAAGGCGGTGGATGA | CCAATCCACAACCAAAATCC | 58 | 192 | 2.2 |
| SlitOR34 | CGCAATATGGGTGTCTTCCT | CATGTTGCTCGATTCCCTTT | 62 | 178 | 2.3 |
| SlitGR1 | CGACATTTACCGCGAATTTT | TTGGGACGAGCCTCAATTAC | 60 | 114 | 2.1 |
| SlitGR2 | GCCGGTGTCCAAGATACACT | CATGCTGATTGCCGAAGTAA | 62 | 168 | 2.2 |
| SlitGR4 | ATGCTGCGTCACACGACTAC | CCAACGGGAACATCTTCAAT | 58 | 115 | 2.2 |
| SlitGR5 | GTTTGTGTTGCTGGTGATGG | TTCGAGGCTAGGATCAAGGA | 62 | 159 | 1.9 |
| S. littoralis RpL8 | ATGCCTGTGGGTGCTATGC | TGCCTCTGTTGCTTGATGGTA | 58/62 | 189 | 1.90 |