Figure 2.

Bradykinin induces elevations in intracellular Ca²⁺ in A498 cells.

Notes: Cells grown on collagen-coated cover slips were incubated with the calcium-sensitive probe Fluo-4AM, and the detection of calcium-dependent fluorescence was performed with a confocal microscope as described in the Materials and methods section. Changes in fluorescence (intracellular free calcium) were recorded as changes in gray-scale intensity. (A) Shown are representative tracings from two recordings in the presence or absence of the BK B₂ receptor antagonist HOE140 (1 μM). (B) Average values of percentage increase over basal in grey-scale intensity subsequent to the addition of BK (100 nM) or the calcium ionophore A23187 (3 μM). Experiments were performed three times.

Abbreviation: BK, bradykinin; RFU, relative fluorescence units.