Fig 2.

Phylogenetic study of Babesia hongkongenesis. (A) Phylogenetic analysis of nucleotide sequences of the 1,368-bp fragment of the 18S rRNA gene of Babesia hongkongensis sp. nov. identified from a free-roaming cat in the present study. The tree was constructed by the neighbor-joining method using Kimura-2 correction and bootstrap values calculated from 1,000 trees. The scale bar indicates the estimated number of substitutions per 50 nucleotides. Plasmodium falciparum (HQ283212.1) was used as the outgroup. (B) Phylogenetic analysis of nucleotide sequences of the 364-bp fragment of the mitochondrial cytochrome b gene of Babesia hongkongensis sp. nov. identified from the free-roaming cat in the present study. The tree was constructed by the neighbor-joining method using Kimura-2 correction and bootstrap values calculated from 1,000 trees. The scale bar indicates the estimated number of substitutions per 20 nucleotides. Plasmodium malariae (GQ355485.1) was used as the outgroup.