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Specific duplex PCR assays for detection of F. hepatica or F. gigantica, using DNA material from eggs from feces of ruminants (A) and humans (B), collected in a province where infection with Fasciola sp. is endemic. Lanes: M, molecular size marker (DNA of λ phage cut by HindIII); (−), negative control (no DNA); (+) Fh and (+) Fg, F. hepatica and F. gigantica positive controls. Samples from humans in panel B are from each individual stool sample.
