Table 1.
Fasciola and platyhelminth species providing DNA template used in the assessment of the specificity and sensitivity for duplex PCR of F. hepatica and F. gigantica
| Sample no. | Sample name | Country where collecteda | Species | Life formb | Host | Reference |
|---|---|---|---|---|---|---|
| 1 | FspHU | Vietnam (HU) | F. gigantica | Adult | Human | This study |
| 2 | FgHaTiB | Vietnam (HT) | F. gigantica | Adult | Cattle | 37 |
| 3 | FspCB1 | Vietnam (CB) | F. gigantica | Adult | Cattle | 22 |
| 4 | FgNBD | Vietnam (NB) | F. gigantica | Adult | Goat | 37 |
| 5 | FhAUS | Australia | F. hepatica | Adult | Cattle | 22 |
| 6 | FspPT | Vietnam (PT) | F. gigantica | Adult | Buffalo | This study |
| 7 | FspBD | Vietnam (BD) | F. gigantica | Adult | Buffalo | This study |
| 8 | FspYB | Vietnam (YB) | F. gigantica | Adult | Goat | 37 |
| 9 | FspPY | Vietnam (PY) | F. gigantica | Adult | Cattle | 22 |
| 10 | FCBB | Vietnam (CB) | F. gigantica | Adult | Cattle | 37 |
| 11 | FspEQB | Ecuador | F. hepatica | Adult | Cattle | This study |
| 12 | FspX | Vietnam (SG) | F. gigantica | Miracidium | Human | 22 |
| 13 | FhETB | Ethiopia | F. hepatica | Adult | Cattle | This study |
| 14 | FNTD2 | Vietnam (NT) | F. gigantica | Adult | Goat | 37 |
| 15 | FNTO1 | Vietnam (NT) | F. gigantica | Adult | Sheep | 37 |
| 16 | FspB3 | Vietnam (HU) | F. gigantica | Adult | Cattle | 22 |
| 17 | FNTO2 | Vietnam (NT) | F. gigantica | Adult | Sheep | 37 |
| 18 | FNTD4 | Vietnam (NT) | F. gigantica | Adult | Goat | This study |
| 19 | FNTD6 | Vietnam (NT) | F. gigantica | Adult | Goat | 37 |
| 20 | FNTD8 | Vietnam (NT) | F. gigantica | Adult | Goat | 37 |
| 21 | FNTS8 | Vietnam (NT) | F. gigantica | Adult | Sheep | 37 |
| 22 | FNTD9 | Vietnam (NT) | F. gigantica | Adult | Goat | 37 |
| 23 | FspT4 | Vietnam (HU) | F. gigantica | Adult | Buffalo | 22 |
| 24 | FhBe | Belgium | F. hepatica | Adult | Cattle | 37 |
| 25 | FNTD7 | Vietnam (NT) | F. gigantica | Adult | Goat | This study |
| 26 | FspBD1 | Vietnam (BD) | F. gigantica | Adult | Human | 22 |
| 27 | FgQN2 | Vietnam (QN) | F. gigantica | Adult | Cattle | This study |
| 28 | FgigTL | Thailand | F. gigantica | Adult | Cattle | This study |
| 29 | FhAUS | Australia | F. hepatica | Eggs | This study | |
| 30 | FspCB1 | Vietnam (NB) | F. gigantica | Eggs | This study | |
| 31 | FspT4 | Vietnam (HU) | F. gigantica | Eggs | This study | |
| 32 | FgigTL | Thailand | F. gigantica | Eggs | This study | |
| 33 | CsND | Vietnam (ND) | Clonorchis sinensis | Adult | Human | 23 |
| 34 | OvBD | Vietnam (BD) | Opisthorchis viverrini | Adult | Human | 23 |
| 35 | HpMcND | Vietnam (ND) | Haplorchis pumilio | Adult | Human | This study |
| 36 | HTA1 | Vietnam (ND) | Haplorchis taichui | Adult | Human | This study |
| 37 | SfQN1 | Vietnam (QN) | Stellantchasmus falcatus | Adult | Human | This study |
| 38 | EcPT | Vietnam (PT) | Echinochasmus japonicus | Adult | Human | This study |
| 39 | FbN | Vietnam (HT) | Faciolopsis buski | Adult | Human | 24 |
| 40 | TsoVN | Vietnam (BN) | Taenia solium | Adult | Human | This study |
a
Capital letters in parentheses indicate provinces or cities in Vietnam where samples were isolated: HU, Hue city; HT, Ha Tay; CB, Cao Bang; NB, Ninh Binh; PT, Phu Tho; YB, Yen Bai; PY, Phu Yen; SG, Ho Chi Minh City; NT, Ninh Thuan; BD, Binh Dinh; QN, Quang Nam; ND, Nam Dinh; BN, Bac Ninh.
b
All eggs were squeezed from the uterus of an adult fluke.