Table 1.
Primers and probes used in this study
| Primer | Sequence (5′–3′) | Amplicon |
|---|---|---|
| Fil_COIclon_fwd | ATTGGTGGTTTTGGTAATTGGATGTTG | First-round PCR for D. immitis, 634 bp |
| Fil_COIclon_R | CAGAAGTCCCCAATACAGCAATCC | |
| COIfel_fwd | GGGTCCTGGGAGTAGTTGAAC | Second-round PCR for D. immitis, 406 bp |
| COIfel_R | TTCACTAACAATCCCAAACACCG | |
| Wol1_fwd | CCTGTACTATATCCAAGAATTACTG | First-round PCR for Wolbachia, 267 bp |
| Wol1_R | ACTATCCTTTATATGTTCCATAATTTC | |
| Wol7_fwd | GGTGGAAATGCTGTGAATAAC | Second-round PCR for Wolbachia, 147 bp |
| Wol7_R | AGCACCGAGCCCTTTAG | |
| Wolb3_fwd | TTGTTGTAGCAAATACGGATG | Real-time PCR, 127 bp |
| Wolb3_R | GCTGCACCTTTACCAATATC | |
| WOLB_TQ3 | [6FAM]AAGGCACCAGCACCGAGC[BHQ1] | |
| F_wol_is1 | ACTATATCCAAGAATTACTGTTGCGTTGTTGATGG | Mimic for first-round PCR for Wolbachia, 220 bp |
| R_wol_is1 | CTTTATATGTTCCATAATTTCATGCTGATACAATAAAGG | |
| F_wol_is7 | GGAAATGCTGTGAATAACACCACTGGTATTGTCATGGACTCTG | Mimic for first-round PCR for Wolbachia, 309 bp |
| R_wol_is7 | CACCGAGCCCTTTAGGCTCTTCTCCAGGGAGGACGA | |
| Fwd_Fil_COIclon_is | TGGTTTTGGTAATTGGATGTTGTCCTGGGAGTAGTTGAAC | Mimic for first-round PCR for D. immitis, 280 bp |
| R_Fil_COIclon_is | AGTCCCCAATACAGCAATCCCTAACAATCCCAAACACCG | |
| Fwd_COIfel_is | TCCTGGGAGTAGTTGAACATGATCTGTCTCTCTTTTCTCCCC | Mimic for second-round PCR for D. immitis, 238 bp |
| R_Fil_COIfel_is | CTAACAATCCCAAACACCGGTACACAAAAAGGTTACATGGAAAGC |