Table 2.
Identification of 18 C. tuberculostearicum strains by 16S rRNA gene sequencing, 18 strains by the API Coryne System,a and 12 strains by MALDI-TOF MS
| No. of strains | 16S rRNA gene sequencing | API Coryne |
Avg MALDI-TOF MS scoreb ± SD |
||||
|---|---|---|---|---|---|---|---|
| Numerical code | First choice listed (% IDd probability, T value) | ID interpretation | Normal protocol | Short extraction protocol | Full extraction protocol | ||
| 5 | C. tuberculostearicum | 4100305 | C. macginleyi (85.6, 0.79) | Good ID on genus level | 1.644 ± 0.31 | 1.917 ± 0.08 | 2.045 ± 0.07 |
| 2 | C. tuberculostearicum | 4100304 | C. jeikeium (59.2, 0.64) | Acceptable ID on genus level | NDc | ND | ND |
| 2 | C. tuberculostearicum | 5100304 | C. macginleyi (97.5, 0.79) | Good ID | 1.486 ± 0.05 | 1.863 ± 0.14 | 2.076 ± 0.10 |
| 2 | C. tuberculostearicum | 7100305 | CDC group G (83, 0.86) | Very good ID on genus level | 1.715 ± 0.37 | 1.82 ± 0.24 | 2.007 ± 0.11 |
| 2 | C. tuberculostearicum | 7100304 | CDC group G (43.5, 0.69) | Good ID on genus level | 1.633 ± 0.12 | 1.894 ± 0.04 | 1.955 ± 0.02 |
| 1 | C. tuberculostearicum | 6100305 | CDC group G (96.7, 0.98) | Good ID | 1.893 | 1.802 | 1.85 |
| 1 | C. tuberculostearicum | 2100305 | CDC group G (86.9, 1) | Excellent ID on genus level | ND | ND | ND |
| 1 | C. tuberculostearicum | 0100305 | C. macginleyi (84.3, 0.82) | Very good ID on genus level | ND | ND | ND |
| 1 | C. tuberculostearicum | 4000304 | C. accolens (90.5, 0.64) | Doubtful profile | ND | ND | ND |
| 1 | C. tuberculostearicum | 6100304 | C. jeikeium (60, 0.79) | Low selectivity | ND | ND | ND |
a
C. tuberculostearicum is not included in the API Coryne database.
b
Interpretation of score values: ≥2.300, highly probable identification to the species level; 2.000 to 2.299; probable identification to the species level, 1.700 to 1.999; probable identification to the genus level; <1.700, no reliable identification.
c
ND, not done.
d
ID, identification.