Table 1.

Performance of EasyQ KPC for detection of bla_KPC genes in 111 KPC-producing and 189 non-KPC-producing Klebsiella pneumoniae and Escherichia coli isolates^a

Microorganism (nb)	No. of KPC-positive isolates identified by EasyQ as:		No. of KPC-negative isolates identified by EasyQ as:		% sensitivity	% specificity
	KPC positive	KPC negative	KPC positive	KPC negative
Reference isolates	18	0	0	11	100	100
Escherichia. colic (1)	0	0	0	1	NDe	ND
Klebsiella pneumoniaed (28)	18	0	0	10	100	100
Bloodstream isolates	93	0	0	178
Escherichia colif (142)	2	0	0	140	100	100
Klebsiella pneumoniaeg (129)	91	0	0	38	100	100
Total (300)	111	0	0	189	100	100

^aIsolates were classified as KPC positive when bla_KPC was identified by standard DNA sequence analysis.

^bn, no. of isolates.

^cE. coli with NDM-1 carbapenemase.

^dEighteen K. pneumoniae isolates were KPC producers: 14 (9 with KPC-2 and 5 with KPC-3) belonged to sequence type (ST) 258, 3 with KPC-3 belonged to ST 512, and one with KPC-2 belonged to ST 101, respectively. Ten KPC-negative isolates produced the VIM-1 (n = 9) or OXA-48 (n = 1) carbapenemase, respectively.

^eND, not determined. Sensitivity was not calculated when <5 isolates were found.

^fE. coli isolates with KPC-3 (n = 2), CTX-M-1 (n = 1), CTX-M-1/SHV-12 (n = 4), CTX-M-3 (n = 1), CTX-M-10 (n = 2), CTX-M-15 (n = 98), CTX-M-15/SHV-12 (n = 12), SHV-2 (n = 1), and SHV-12 (n = 21).

^gK. pneumoniae isolates with KPC-3 (n = 91), CTX-M-15 (n = 12), SHV-5 (n = 4), and SHV-12 (n = 22).