Table 1.
Identification of 659 Gram-positive rods using the MALDI-TOF MS Andromas systema
Genus or species | No. of isolates tested | Identification result (no. of isolates) |
|||
---|---|---|---|---|---|
Species level | Genus level | Not identified | Error | ||
Actinobaculum spp.b | 18 | 18 | 0 | 0 | 0 |
A. schaalii | 14 | 14 | 0 | 0 | 0 |
A. massiliense | 4 | 4 | 0 | 0 | 0 |
Actinomyces spp.b | 76 | 75 | 1 | 0 | 0 |
A. europaeus | 1 | 1 | 0 | 0 | 0 |
A. funkei | 4 | 4 | 0 | 0 | 0 |
A. naeslundii | 1 | 1 | 0 | 0 | 0 |
A. neuii | 33 | 33 | 0 | 0 | |
A. odontolyticus | 5 | 5 | 0 | 0 | 0 |
A. radingae | 6 | 6 | 0 | 0 | 0 |
A. turicensis | 23 | 23 | 0 | 0 | 0 |
A. urogenitalis | 2 | 2 | 0 | 0 | 0 |
A. viscosus | 1 | 0 | 1 | 0 | 0 |
Bacillus spp.b | 21 | 21 | 0 | 0 | 0 |
B. cereus/B. thuringiensis | 10 | 10 | 0 | 0 | 0 |
B. licheniformis | 2 | 2 | 0 | 0 | 0 |
B. pumilus/B. safensis | 4 | 4 | 0 | 0 | 0 |
B. subtilis | 3 | 3 | 0 | 0 | 0 |
B. simplex | 2 | 2 | 0 | 0 | 0 |
Corynebacterium spp.b | 342 | 338 | 2 | 1 | 1 |
C. amycolatum | 97 | 97 | 0 | 0 | 0 |
C. aurimucosum | 30 | 27 | 2 | 0 | 1 |
C. coyleae | 3 | 3 | 0 | 0 | 0 |
C. diphtheriae | 40 | 39 | 0 | 1 | 0 |
C. glucuronolyticum | 10 | 10 | 0 | 0 | 0 |
C. imitans | 2 | 2 | 0 | 0 | 0 |
C. jeikeium | 16 | 16 | 0 | 0 | 0 |
C. massiliensis | 1 | 1 | 0 | 0 | 0 |
C. pseudodiphtheriticum | 10 | 10 | 0 | 0 | 0 |
C. pseudotuberculosis | 13 | 13 | 0 | 0 | 0 |
C. simulans | 25 | 25 | 0 | 0 | 0 |
C. singulare | 1 | 1 | 0 | 0 | 0 |
C. striatum | 44 | 44 | 0 | 0 | 0 |
C. tuberculostearicum | 18 | 18 | 0 | 0 | 0 |
C. ulcerans | 19 | 19 | 0 | 0 | 0 |
C. urealyticum | 10 | 10 | 0 | 0 | 0 |
Lactobacillus spp.b | 30 | 30 | 0 | 0 | 0 |
L. casei | 1 | 1 | 0 | 0 | |
L. crispatus | 8 | 8 | 0 | 0 | 0 |
L. delbrueckii | 1 | 1 | 0 | 0 | 0 |
L. fermentum | 2 | 2 | 0 | 0 | 0 |
L. gasseri | 7 | 7 | 0 | 0 | 0 |
L. jensenii | 6 | 6 | 0 | 0 | 0 |
L. rhamnosus | 5 | 5 | 0 | 0 | 0 |
Listeria spp.b | 56 | 4 | 52 | 0 | 0 |
L. grayi | 4 | 4 | 0 | 0 | |
L. innocua | 5 | 0 | 5 | 0 | 0 |
L. ivanovii | 5 | 0 | 5 | 0 | 0 |
L. monocytogenes | 32 | 0 | 32 | 0 | 0 |
L. seeligeri | 5 | 0 | 5 | 0 | 0 |
L. welshimeri | 5 | 0 | 5 | 0 | 0 |
Nocardia spp.b | 46 | 42 | 4 | 0 | 0 |
N. abscessus | 4 | 4 | 0 | 0 | 0 |
N. asteroides | 2 | 2 | 0 | 0 | 0 |
N. arthritidis | 1 | 0 | 1 | 0 | 0 |
N. beijingensis | 3 | 3 | 0 | 0 | 0 |
N. brasiliensis | 7 | 6 | 1 | 0 | 0 |
N. cyriacigeorgica | 7 | 6 | 1 | 0 | 0 |
N. farcinica | 11 | 10 | 1 | 0 | 0 |
N. nova | 5 | 5 | 0 | 0 | |
N. mexicana | 1 | 1 | 0 | 0 | 0 |
N. otitidiscavium | 2 | 2 | 0 | 0 | 0 |
N. paucivorans | 1 | 1 | 0 | 0 | 0 |
N. veterana | 2 | 2 | 0 | 0 | 0 |
Propionibacterium spp.b | 29 | 29 | 0 | 0 | 0 |
P. acnes | 11 | 11 | 0 | 0 | 0 |
P. avidum | 18 | 18 | 0 | 0 | 0 |
Other GPR speciesc | 41 | 41 | 0 | 0 | 0 |
Arthrobacter cumminsii | 1 | 1 | 0 | 0 | 0 |
Brevibacterium casei | 3 | 3 | 0 | 0 | 0 |
Brevibacterium paucivorans | 1 | 1 | 0 | 0 | 0 |
Dermabacter hominis | 12 | 12 | 0 | 0 | 0 |
Erysipelothrix rhusiopathiae | 2 | 2 | 0 | 0 | 0 |
Gardnerella vaginalis | 3 | 3 | 0 | 0 | 0 |
Paenibacillus motobuensis | 1 | 1 | 0 | 0 | 0 |
Rhodococcus equi | 2 | 2 | 0 | 0 | 0 |
Turicella otitidis | 16 | 16 | 0 | 0 | 0 |
The mass spectra of the isolates studied were compared with those of the whole Andromas database, which encompasses more than 700 bacterial species, including 33 genera and 156 species of aerobically growing Gram-positive rods (GPRs).
For these genera, the total numbers of taxa included in the Andromas database are 18 Actinomyces, 3 Actinobaculum, 13 Bacillus, 32 Corynebacterium, 24 Lactobacillus, 6 Listeria, 25 Nocardia, and 4 Propionibacterium species.
For these genera, the total numbers of taxa included in the Andromas database are 5 Arthrobacter, 6 Brevibacterium, 1 Dermabacter, 1 Erysipelothrix, 1 Gardnerella, and 3 Paenibacillus species, 1 Rhodococcus species, and 1 Turicella species.