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Performance of different methods
| Methoda | No. of samples |
No. of positive PCRs by replicate |
||||
|---|---|---|---|---|---|---|
| Negative | Positive | % Positive | 1 | 2 | 3 | |
| IHE-PF | 487 | 12 | 2.4 | 10 | 1 | 1 |
| IHE-HHQ | 483 | 16 | 3.2 | 14 | 1 | 1 |
| UCP-PF | 470 | 29 | 5.8 | 17 | 5 | 7 |
| UCP-HHQ | 470 | 27 | 5.4 | 14 | 4 | 9 |
| GM | 486 | 7 | 1.4 | |||
The eluates of two different extraction methods (IHE and UCP) were tested in crossover experiments by two different real-time PCR assays (PF and HHQ). GM represents the results for the galactomannan ELISA.
