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. 2012 Aug;50(8):2668–2673. doi: 10.1128/JCM.00457-12

Fig 4.

Fig 4

Optimization of PCR using individual tissue DNA with individual multiplex PCR primers. Lanes: 1, 1-kb DNA ladder; 2, ALV-positive cell culture DNA showing an amplicon size of 613 bp with p27 gene primers; 3, MDV-positive cell culture DNA showing an amplicon size of 856 bp with meq gene primers; 4, β-actin gene-specific primers used as internal control showing an amplicon size of 396 bp in CEF cell culture DNA; 5, REV-positive cell culture DNA showing an amplicon size of 204 bp with LTR sequence-specific primers.