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. 2012 Aug;50(8):2668–2673. doi: 10.1128/JCM.00457-12

Fig 6.

Fig 6

Sensitivity of multiplex PCR primers using PCR product cloned TA plasmid DNA. (a) The meq gene primer amplicon (856 bp) was seen in plasmid copy numbers of 514 (lane 1) and up to 52 in serial dilution (lanes 3 to 14). (b) The ALV p27 gene primer amplicon (613 bp) was seen in copy numbers of 515 (lane 1) and up to 53 in serial dilution (lanes 3 to 14). (c) The REV LTR sequence primer amplicon (204 bp) was seen in copy numbers of 515 (lane 1) and up to 53 in serial dilution (lanes 3 to 14). Lane 2, nontemplate control.