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SCGE-based PCR ribotyping results for C. difficile reference strain UK6 (PCR ribotype 027 [8]). (A, B, and C) SCGE-based PCR ribotyping amplified by primer pairs 16S-USA/23S-USA, 16S-UK/23S-UK, and 16S-UK/23S-AU, respectively. The sizes of peaks a to h are shown in Table 3. SCGE was performed on an AB 3730xl DNA analyzer (Applied Biosystems) with the 20- to 1,200-bp LIZ 1200 ladder as the internal size control.
