Table 1.
Polymorphisms in the MDR1 promoters of clinical C. albicans isolates
| Isolate | Allele(s) | Nucleotidea at position: |
||||
|---|---|---|---|---|---|---|
| −343 | −306 | −154 | −152 | −137 | ||
| F2 (Flus)b | 1 | T | G | A | T | A |
| 2 | A | G | A | T | A | |
| F5 (Flur)b | 1 | T | G | A | T | A |
| 2 | A | G | A | T | A | |
| G2 (Flus)b | 1 | T | G | A | T | A |
| 2 | A | G | A | T | A | |
| G5 (Flur)b | 1 | T | G | A | T | A |
| 2 | A | G | A | T | A | |
| B3 (Flus)c | 1, 2 | T | A | G | A | C |
| B4 (Flur)c | 1, 2 | T | A | G | A | C |
| 5044 (Flus)c | 1, 2 | A | G | A | T | A |
| 5052 (Flur)c | 1, 2 | A | G | A | T | A |
| 1442 (Flus)c | 1, 2 | A | A/G | A/G | A/T | A/C |
| 2271 (Flur)c | 1, 2 | A | A/G | A/G | A/T | A/C |
| 5833 (Flus)c | 1, 2 | A | A/G | A/G | A/T | A/C |
| 6692 (Flur)c | 1, 2 | A | A/G | A/G | A/T | A/C |
| 1490 (Flus)c | 1, 2 | T | A/G | A/G | A/T | A/C |
| 1587 (Flur)c | 1, 2 | T | A/G | A/G | A/T | A/C |
| DSY291 (Flus)c | 1, 2 | T | A | A/G | A/T | A/C |
| DSY292 (Flur)c | 1, 2 | T | A | A/G | A/T | A/C |
| DSY2285 (Flus)c | 1, 2 | T | G | A/G | A/T | A/C |
| DSY2286 (Flur)c | 1, 2 | T | G | A/G | A/T | A/C |
| Signature | A type | T | A | G | A | C |
| Signature | G type | A | G | A | T | A |
a
Only the diagnostic nucleotides that define A- and G-type alleles, named after the nucleotide at position −306 (gray column) are shown. The signature sequences of A-type and G-type alleles are shown at the bottom.
b
The sequences of both MDR1 promoter alleles of these isolates were determined in a previous study (31).
c
The MDR1 promoters of these isolates were amplified by PCR, followed by direct sequencing of the PCR products. Polymorphic nucleotides could therefore not be assigned to one or the other allele.