Table 1.
Typical growth parameters, antifungal MIC, rhodamine 6G efflux, and sterol data for C. glabrata study isolates cultured using YEPDa
| Isolate | Growth parameter |
MIC (μg ml−1) |
R6G assay efflux ratio | Sterol distribution (%) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ΔOD600 | Lag phase (h) | T half max (h) | Max DT (h) | FLC | VRC | AMB | Lanosterol | 14α-Methylatedb | Ergosterol | 14α-Demethylatedc | ||
| CG26 | 1.30 | 6.3 | 9.0 | 1.0 | 256 | 16 | 2 | 12.66 | 4.0 | — | 80.5 | 15.5 |
| CG44 | 1.32 | 6.5 | 9.5 | 1.0 | 128 | 2 | 2 | 1.2 | 3.6 | — | 75.5 | 20.9 |
| CG388 | 1.49 | 6.0 | 8.0 | 1.0 | 128 | 2 | 2 | 2.7 | 2.5 | — | 82.7 | 14.8 |
| CG1012 | 1.55 | 6.0 | 8.5 | 1.0 | 128 | 2 | 2 | 2.5 | 3.3 | — | 77.6 | 19.1 |
| CG156 | 1.34 | 14.5 | 18.5 | 2.0 | >256 | >256 | 32 | 1.5 | 81.5 | 18.5 | — | — |
a
ΔOD600, maximum minus minimum optical density reading (at 600 nm); lag phase, length of time culture remains at <10% of maximum OD; T half max, time taken to achieve half-maximal culture growth [(maximum OD minus minimum OD) times 0.5]; max DT, maximum observed doubling time; FLC, fluconazole; VRC, voriconazole; AMB, amphotericin B. R6G efflux values indicate ratios of fluorescence to forward scatter (FSC/FL-2) for 1.2 × 104 cells. The highest values are in boldface; —, not detected.
b
Other (not lanosterol) 14α-methylated sterols.
c
Other (not ergosterol) 14α-demethylated sterols.