Table 4.
Sterol composition of CG156 and CG388 after 48 h culture in glcYMa
| Strain and parameter | Sterol composition (%) in glcYM with: |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No supplement |
7,22 |
ERG |
LAN |
CAN |
CER |
CEN |
DES |
|||||||||
| FLC− | FLC+ | FLC− | FLC+ | FLC− | FLC+ | FLC− | FLC+ | FLC− | FLC+ | FLC− | FLC+ | FLC− | FLC+ | FLC− | FLC+ | |
| CG156 | ||||||||||||||||
| Cholesterol | [2.8] | [2.0] | 30.7 | 27.8 | ||||||||||||
| Δ7-Cholestenol (lathosterol) | 1.6 | 9.5 | ||||||||||||||
| Cholestanol | 19.6 | 22.7 | ||||||||||||||
| Desmosterol | 2.8 | 1.9 | ||||||||||||||
| Ergosta 5,22-dienol | [16.3] | [15.4] | ||||||||||||||
| Zymosterol | ||||||||||||||||
| Ergosterol | [14.3] | [12.8] | 25.2 | 29.2 | ||||||||||||
| Ergosta 7,22-dienol | 0.8 | 0.3 | ||||||||||||||
| Ergosta 5-enol | [10.7] | [5.7] | ||||||||||||||
| 14α-Methyl fecosterol | 3.4 | 3.6 | 1.7 | 3.9 | 1.2 | 3.6 | 1.4 | 4.4 | 1.3 | 1.5 | 2.3 | 3.8 | 1.2 | 2.2 | 1.5 | 3.6 |
| Fecosterol | ||||||||||||||||
| Ergosta 8-enol | ||||||||||||||||
| 4,14α-Dimethylzymosterol | 11.5 | 12.1 | 4.4 | 6.1 | 2.3 | 2.9 | 7.0 | 10.3 | 2.7 | 4.9 | 1.4 | 3.5 | 6.4 | 8.7 | 7.4 | 4.9 |
| Ergosta 5,7-dienol | ||||||||||||||||
| Episterol | ||||||||||||||||
| Ergosta 7-enol | ||||||||||||||||
| 14α-Methyl 3β,6α-diolb | 7.5 | 8.2 | 1.6 | 5.2 | 0.7 | 1.5 | 4.8 | 7.6 | 0.8 | 1.5 | 1.0 | 2.8 | 2.6 | 1.7 | 1.9 | 1.5 |
| Lanosterol | 74.0 | 71.1 | 75.8 | 69.5 | 70.0 | 60.9 | 85.3 | 77.6 | 71.1 | 66.7 | 62.7 | 59.8 | 86.3 | 76.6 | 59.4 | 66.3 |
| 4,4-Dimethylzymosterol | ||||||||||||||||
| Eburicol | 0.7 | 1.2 | 0.2 | 1.1 | 0.8 | 0.4 | 1.0 | 1.2 | 0.6 | 0.6 | ||||||
| Unknown | 3.5 | 5.0 | 0.7 | 0.9 | 0.4 | 0.8 | 1.5 | 0.8 | 0.4 | 1.0 | 1.0 | 1.3 | 1.4 | 0.2 | ||
| CG388 | ||||||||||||||||
| Cholesterol | [3.2] | 35.3 | ||||||||||||||
| Δ7-Cholestenol (lathosterol) | 8.5 | |||||||||||||||
| Cholestanol | 22.7 | |||||||||||||||
| Desmosterol | 2.2 | |||||||||||||||
| Ergosta 5,22-dienol | [16.7] | |||||||||||||||
| Zymosterol | 3.7 | 0.7 | 3.6 | 0.8 | 4.0 | 3.2 | 0.6 | 4.2 | 4.0 | 3.3 | 1.7 | 4.5 | ||||
| Ergosterol | 59.7 | 37.0 | 60.4 | 45.5 | 63.1 | 43.3 | 65.4 | 1.7 | 61.7 | 0.9 | 60.7 | 3.3 | 63.4 | 1.3 | 62.0 | 7.9 |
| Ergosta 7,22-dienol | 7.6 | 7.0 | 0.7 | 6.7 | 0.8 | 6.3 | 6.4 | 7.2 | 7.7 | 7.5 | ||||||
| Ergosta 5-enol | [8.7] | |||||||||||||||
| 14α-Methyl fecosterol | 1.0 | 0.3 | 1.5 | 0.5 | 0.5 | 0.9 | 2.3 | 0.4 | ||||||||
| Fecosterol | 3.2 | 3.5 | 2.4 | 2.0 | 2.8 | 3.1 | 2.1 | 2.2 | ||||||||
| Ergosta 8-enol | 1.7 | 2.0 | 1.5 | 1.6 | 2.0 | 2.0 | 1.9 | 2.2 | ||||||||
| 4,14α-Dimethylzymosterol | 2.0 | 1.1 | 1.5 | 3.7 | 6.4 | 4.4 | 9.1 | 4.3 | ||||||||
| Ergosta 5,7-dienol | 5.2 | 5.5 | 5.8 | 4.7 | 5.0 | 5.1 | 5.4 | 5.7 | ||||||||
| Episterol | 6.1 | 5.8 | 6.4 | 6.1 | 6.1 | 6.0 | 5.8 | 6.0 | ||||||||
| Ergosta 7-enol | 1.2 | 1.6 | 1.4 | 1.6 | 1.3 | 1.2 | 1.1 | 1.5 | ||||||||
| 14α-Methyl 3β,6α-diol | 1.5 | 1.0 | 1.1 | 3.8 | 2.5 | 3.2 | 4.1 | 1.6 | ||||||||
| Lanosterol | 2.3 | 55.1 | 2.6 | 47.8 | 2.2 | 50.1 | 2.5 | 89.7 | 3.0 | 63.9 | 3.3 | 52.5 | 3.0 | 73.0 | 2.5 | 57.3 |
| 4,4-Dimethylzymosterol | 9.4 | 1.9 | 8.0 | 6.5 | 6.6 | 7.5 | 7.4 | 6.3 | 5.9 | |||||||
| Eburicol | 1.9 | 1.3 | 0.2 | 0.4 | ||||||||||||
| Unknown | 0.0 | 0.7 | 0.8 | 0.5 | 0.2 | 0.3 | ||||||||||
a
The most abundant sterol in each treatment is in boldface; exogenously supplied sterols are underlined; metabolites of exogenously supplied sterols are in brackets. FLC was added at ×0.5 MIC.
b
14α-Methyl ergosta 8,24(28)-dien-3β-6α-diol is a fungistatic sterol (16).