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. 1978 Jul;5(7):2289–2296. doi: 10.1093/nar/5.7.2289

Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-(aminomethyl)-7-deazaguanosine.

N Okada, S Noguchi, S Nishimura, T Ohgi, T Goto, P F Crain, J A McCloskey
PMCID: PMC342163  PMID: 353740

Abstract

A precursor of modified nucleoside Q isolated from E. coli methyl-deficient tRNA was determined to be 7-(aminomethyl)-7-deazaguanosine. The structure was deduced by means of its chromatographic and electrophoretic mobilities, and UV and mass spectra, in addition to comparison with the synthesized authentic compound. The same molecule is also found in tRNA of an E. coli mutant selected for deficient synthesis of modified nucleosides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Isono K., Krauss J., Hirota Y. Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins. Mol Gen Genet. 1976 Dec 22;149(3):297–302. doi: 10.1007/BF00268531. [DOI] [PubMed] [Google Scholar]
  2. Kasai H., Kuchino Y., Nihei K., Nishimura S. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's. Nucleic Acids Res. 1975 Oct;2(10):1931–1939. doi: 10.1093/nar/2.10.1931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kasai H., Oashi Z., Harada F., Nishimura S., Oppenheimer N. J., Crain P. F., Liehr J. G., von Minden D. L., McCloskey J. A. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine. Biochemistry. 1975 Sep 23;14(19):4198–4208. doi: 10.1021/bi00690a008. [DOI] [PubMed] [Google Scholar]
  4. McCloskey J. A., Lawson A. M., Tsuboyama K., Krueger P. M., Stillwell R. N. Mass spectrometry of nucleic acid components. Trimethylsilyl derivatives of nucleotides, nucleosides, and bases. J Am Chem Soc. 1968 Jul 17;90(15):4182–4184. doi: 10.1021/ja01017a062. [DOI] [PubMed] [Google Scholar]
  5. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  6. Okada N., Yasuda T., Nishimura S. Detection of nucleoside Q precursor in methyl-deficient E.coli tRNA. Nucleic Acids Res. 1977 Dec;4(12):4063–4075. doi: 10.1093/nar/4.12.4063. [DOI] [PMC free article] [PubMed] [Google Scholar]

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