Fig 1.

Full-length Oas1b contains a C-terminal transmembrane domain that localizes it to the ER membrane. (A) Schematic representation of the full-length Oas1b protein (product of the Flv resistance allele), Oas1bΔTM (a constructed deletion mutant that lacks the C-terminal transmembrane tail), and Oas1bt (product of the Flv susceptibility allele that has a premature stop codon). aa, amino acids. (B) Plasmids expressing GST, GST-Oas1bt, or GST-Oas1b were transfected into BHK cells, and 24 h later cells were either mock infected or infected with WNV Eg101 (MOI of 5). At 24 h after infection, cells were fixed, permeabilized, and analyzed by confocal microscopy. Anti-GST antibody (green) detected GST-tagged proteins, anticalnexin antibody (red) detected ER, anti-CoxIV antibody (red) detected mitochondria, and Hoechst stain (blue) detected nuclei.