Fig 5.

PARP12L and Bst2 expression has a negative effect on VEEV mutant replication. (A) Schematic representation of recombinant viral genomes. HG indicates a position of cloned heterologous genes. (B) Schematic representation of long and short isoforms of PARP12 protein. (C) Replication of recombinant VEEV variants expressing the indicated heterologous genes in NIH 3T3 cells. Cells were infected at an MOI of 10 PFU/cell, media were replaced at the indicated times, and virus titers were determined by plaque assay on BHK-21 cells.