Fig 6.

The antiviral effects of PARP12L and Bst2, expressed from the viral genome, is dependent on the applied MOI and cell type. (A) The schematic representation of recombinant VEEV genomes encoding mutated capsid protein, Bst2 and the PARP12L genes in direct or reverse orientations. (B) NIH 3T3 and BHK-21 cells were infected with recombinant viruses encoding PARP12L, Bst2, or PARP12L in reverse orientation at the indicated MOIs. Media were harvested at the indicated times postinfection, and virus titers were determined by plaque assay on BHK-21 cells.