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. 2012 Aug;86(15):7806–7817. doi: 10.1128/JVI.00080-12

Table A1.

List of parameters used for the simulation of influenza virus replication

Parameter Description Value Source, reference, or comment
Bhitot No. of high-affinity binding sites 150 sites 44
Blotot No. of low-affinity binding sites 1,000 sites 44
DRib Distance between two adjacent ribosomes 160 nt 4
FFus Fraction of fusion-competent virions 0.51 Expt in Fig. 2A
FSp17 Fraction of M2-encoding mRNAs 0.02 Based on ration of M2 to M1
FSp18 Fraction of NEP-encoding mRNAs 0.125 50
khiAtt Attachment to high-affinity binding sites 8.09 · 10−2 sites−1 · h−1 Adjusted to data in reference 44
kloAtt Attachment to low-affinity binding sites 4.55 · 10−2 sites−1 · h−1 Adjusted to data in reference 44
kM1Bind Binding of M1 to nuclear vRNPs 1.39 · 10−6 molecule−1 · h−1 Expt in Fig. 4A and B
kNPBind Binding of NP to RdRp-RNA complexes 3.01 · 10−4 molecule−1 · h−1 Expt in Fig. 3B-D
kRdRpBind Binding of RdRp complexes to RNA 1 molecule−1 · h−1 Expt in Fig. 3B-D
kMDeg Degradation of mRNA 0.33 h−1 Expt in Fig. 3B-D
kRDeg Degradation of nascent cRNA/vRNA 36.36 h−1 Expt in Fig. 3B-D
kRnpDeg Degradation of RNPs 0.09 h−1 Expt in Fig. 4A and B
kRRdRpDeg Degradation of RdRp-RNA complexes 4.25 h−1 Expt in Fig. 3B-D
kEn Endocytosis 4.8 h−1 Expt in Fig. 2A
khiEq Equilibrium constant of high-affinity sites 1.13 · 10−2 site−1 44
kloEq Equilibrium constant of low-affinity sites 8.33 · 10−5 site−1 44
kExp NEP binding and nuclear export 1 · 10−6 molecule−1 · h−1 Adjusted to data from reference 2
kFus Fusion with endosomes 3.21 h−1 Expt in Fig. 2A
kImp Nuclear import 6 h−1 5
kRdRp Formation of RdRp complexes 1 molecule−2 · h−1 Rapid complex formation
kRel Virus release 3.7 · 10−3 virions · molecule−1 · h−1 Cell releases 104 virions in 12 h
kCSyn cRNA synthesis 1.38 h−1 Expt in Fig. 4A and B
kMSyn mRNA synthesis 2.5 · 10−5 nt · h−1 Expt in Fig. 4A and B
kPSyn Protein synthesis 64,800 nt · h−1 56
kVSyn vRNA synthesis 13.86 h−1 Expt in Fig. 4A and B
KVRel Influence of proteins on virus release 10 virions Adjusted
L1 Length of segment 1's mRNA 2,320 nt 33
L2 Length of segment 2's mRNA 2,320 nt 33
L3 Length of segment 3's mRNA 2,211 nt 33
L4 Length of segment 4's mRNA 1,757 nt 33
L5 Length of segment 5's mRNA 1,540 nt 33
L6 Length of segment 6's mRNA 1,392 nt 33
L7 Length of segment 7's unspliced mRNA 1,005 nt 33
L8 Length of segment 8's unspliced mRNA 868 nt 33
LV Average length of a vRNA 1,700 nt Based on reference 33
NPRdRP No. of RdRp complexes in a virion 45 molecules · virion−1 33
NPHA No. of HA molecules in a virion 500 molecules · virion−1 33
NPNP No. of NP molecules in a virion 1,000 molecules · virion−1 33
NPNA No. of NA molecules in a virion 100 molecules · virion−1 33
NPM1 No. of M1 molecules in a virion 3,000 molecules · virion−1 33
NPM2 No. of M2 molecules in a virion 40 molecules · virion−1 33
NPNEP No. of NEP molecules in a virion 165 molecules · virion−1 33
NM1nuc Nucleotides bound by one M1 molecule 200 nt 63
NNEPNuc Nucleotides bound by one NEP molecule 1,700 nt Adjusted to data in reference 47
NNPnuc Nucleotides bound by one NP molecule 24 nt 47