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. 2012 Aug;86(15):7836–7849. doi: 10.1128/JVI.00538-12

Fig 5.

Fig 5

Tethered PABP stimulated cap-independent translation of a reporter RNA1. (A) Schematic representation of the components used for the assay of tethered PABP function. R1-LucΔAms2bs is a reporter RNA1 variant that lacks the ARS but contains tandem binding sites for the MS2 bacteriophage coat protein (MS2CP) in the 3′ UTR. (B) The translational activity of R1-LucΔAms2bs was similar to that of R1-Luc-AC4 (negative control) in BYL. The level of expression of luciferase from R1-Luc-AC4 was defined as 100%. Means ± the SD (n = 3) are shown. (C) The translational activity of R1-LucΔAms2bs was enhanced in the presence of MS2CP-NtPABP in BYL. The level of expression of luciferase from R1-Luc-AC4 was defined as 100%. Means ± the SD (n = 3) are shown. (D) MS2CP-NtPABP specifically enhanced the translational activity of R1-LucΔAms2bs in cis in BYL. The level of expression of luciferase from R1-Luc-R1 (positive control) in BYL expressing NtPABP was defined as 100%. Means ± the SD (n = 3) are shown.