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Stability of a reporter RNA1 (R1-Luc-R1) and its derivatives. (A) BYL was incubated with R1-Luc-R1 and its derivatives. Total RNA was extracted at the indicated time points and used for Northern blotting with the positive-strand detection probe (26). (B) The signal intensities were quantified with Image Gauge program (Fujifilm Life Sciences). Transcripts abundance for each reporter mRNA was normalized to 0.1-min time point and fits to decaying exponential with IGOR pro 6 (Wavemetrics).
