Fig 7.

Both the ARS and 3′CITE are required for the efficient cap- and poly(A)-independent translation in BY-2 and cowpea protoplasts. (A) Schematic representation of introduced mutations in the 5′ UTR, ARS, and 3′CITE of reporter RNA1. (B and C) Nonfunctional-capped (A-capped) R1-Luc-R1 and its derivatives, and functional-capped (m⁷G-capped) and nonfunctional-capped luciferase mRNA with or without poly(A) tail were cotransfected with Renilla luciferase mRNA (internal control) into BY-2 and cowpea protoplasts using PEG transfection regent and electroporation, respectively. The FL/RL luminescence was normalized to the value of Δ5′-Luc-AC4-Lm1. Means ± the SD (n = 3) are shown.