Fig 3.

Intracellular cytokine staining and polyfunctionality of T-cell responses at 2 weeks after the boost. Spleens from mice were harvested and crushed, and then single-cell suspensions were pulsed with the HIV envelope peptide pool. (A) Intracellular cytokine staining (IFN-γ, TNF-α, and IL-2) was performed, and respective frequencies for CD4⁺ and CD8⁺ T cells were determined. (B) Using SPICE software, we calculated and displayed polyfunctionality of the T-cell responses.