Fig 1.

Requirements for suppressing ERK activation in HSV-1-infected cells. (A) Serum-deprived NHDFs were mock infected or infected with HSV-1 or UV-inactivated HSV-1 at a MOI of 5. Total protein was collected at 13 hpi, separated by SDS-PAGE, and analyzed by immunoblotting with the indicated antisera. (B) Same as for panel A, except that total protein was collected at the indicated times postinfection. (C) Same as for panel A, except that cells were mock infected or infected with WT KOS or the ICP4-deficient N12 mutant (MOI = 5). Total protein was collected at 9 hpi and analyzed as described for panel A. (D) Same as for panel A, except that cells were pretreated with ±300 μg/ml phosphonoacetic acid (PAA) for 30 min and then mock infected or infected with HSV-1 (MOI = 5) in the presence or absence of PAA. At 13 hpi, total protein was collected and analyzed as described for panel A.