Fig 2.

Suppression of ERK activation in HSV-1-infected cells is dependent upon the Us3 Ser/Thr kinase. (A) Serum-deprived NHDFs were mock infected or infected with WT HSV-1 or a Us3-deficient mutant (ΔUs3) at a MOI of 5. Total protein was harvested at 15 hpi and analyzed by immunoblotting using the indicated antisera. (B) Same as for panel A, except that cells were infected with a UL13-deficient virus (ΔUL13), a UL13-deficient virus in which the mutation was repaired (ΔUL13-Repair), or WT virus. (C) Samples as described for panels A and B were analyzed by immunoblotting using anti-Us11.