Fig 4.

Us3-dependent suppression of ERK activity does not require Us3-mediated mTORC1 activation. (A) Serum-deprived NHDFs were pretreated with DMSO, 100 nM rapamycin, or 2.5 μM PP242 for 30 min and subsequently mock infected or infected with HSV-1 (MOI = 5) in the presence or absence of inhibitors. At 15 hpi, total protein was isolated and analyzed by immunoblotting with the indicated antisera. (B) NHDFs were transfected with control or nonsilencing (NS) or TSC2 siRNA for 48 h, serum deprived for 72 h, and infected with ΔUS3 or ΔUs3-Repair (MOI = 5) as described previously (7). At 14 hpi, total protein was harvested and analyzed as described for panel A.