Syntenin-2 is downregulated at the transcriptional level in HPV8 E6-expressing cells. (A) Syntenin-1 mRNA expression in keratinocytes retrovirally infected with the empty construct pLXSN or constructs coding for HPV8 E6, E7, or E6E7. (B) Syntenin-2 mRNA expression was quantified in keratinocytes positive for pLXSN, HPV8 E6, E7, or E6E7. (C and D) PHEK expressing HPV8 E6 wt or HPV8 E6Δ132-136 were studied for E6 (C) and for syntenin-2 (D) mRNA and protein expression. (E) MRNA levels of syntenin-2, Dlg1, Scribble, Magi-1 and -3, Mupp1, and PSD95 in pLXSN- or HPV8 E6-positive primary keratinocytes. MRNA levels of gene targets were normalized to HPRT1 mRNA levels. Error bars show standard deviations.