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. 1978 Jul;5(7):2345–2358. doi: 10.1093/nar/5.7.2345

Cross-linking of nucleosomal histones with monofunctional imidoesters.

L Wyns, I Lasters, R Hamers
PMCID: PMC342168  PMID: 27763

Abstract

Cross-linking experiments with the MONOfunctional imidoester methyl-acetimidate, in the pH range 7.0 - 8.0, on rat liver nucleosomes generate a cross-linking pattern almost identical with the one observed for much longer BIfunctional reagents (e.g. dimethylsuberimidate). Combined cross-linking and trypsin digestion experiments suggest that all or at least the great majority of this cross-linking occurs on trypsin digestible segments (or "tails") of the histones. The formation of oligomers over such extremely short distances and especially the observation of an H3 homodimer suggests a very close proximity of half-nucleosomes.

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