Fig 1.

Virus-specific CD8 T cells at day 30 after LCMV clone 13 infection do not persist if adoptively transferred to infection-free recipients. (A) Experimental design. Ly5.1⁺ CD8 T cells were purified from day 30 LCMV clone 13-infected mice and adoptively transferred to day 30 LCMV Arm-immune or day 30 LCMV clone 13-infected recipient mice. Approximately 2 × 10⁵ D^bGP33⁺ CD8 T cells were adoptively transferred to each recipient mouse. Donor virus-specific CD8 T cells were examined on days 2 and 60 p.t. (B) Gating strategy used to identify donor CD8 T cell population. Representative examples of the day 30 clone 13 → Arm and day 30 clone 13 → clone 13 recipient mice on both day 2 p.t. and day 60 p.t. (C) Absolute numbers of D^bGP33⁺ CD8 T cells in the spleens of recipient mice on days 2 and 60 p.t. normalized to the number of cells on day 2 p.t. Each symbol represents one mouse, with red squares signifying antigen-specific CD8 T cells transferred to clone 13-infected recipient mice and blue circles representing cells transferred to day 30 Arm-infected recipient mice. ns, not significant; **, P < 0.01.