Fig 1.

Schematic representation of the bmnpv-miR-1 binding site on Ran mRNA and the luciferase constructs. (A) Ran mRNA showing the putative binding site of bmnpv-miR-1. (B) Luciferase reporter constructs containing the Ran 3′ UTR (as shown in panel A) and the 3′ UTR of the unrelated target PPO (prophenoloxidase) fused at the 3′ end of the firefly luciferase (luc) gene. The firefly and Renilla luciferase genes are driven by human phosphoglycerate kinase (PGK) and simian virus 40 (SV40) promoters, respectively. (C) The WT or the Mut 3′ UTR of Ran encompassing the binding site of bmnpv-miR-1 was cloned into the PmirGLO vector downstream of the firefly luciferase gene (PmirGLO-Ran-WT and PmirGLO-Ran-Mut, respectively). The 4 mutated nucleotides (PmirGLO-Ran-Mut) in the seed region of the bmnpv-miR-1 binding site are highlighted in red.