Fig 4.

bmnpv-miR-1 downregulates Ran expression in B. mori larvae and in BmN cells. (A) RT-qPCR analysis of Ran transcripts in the fat body tissues of B. mori larvae administered bmnpv-miR-1 with or without its specific LNA. Larvae administered DEPC-H₂O were used as a negative control. (B) Western blots showing a decrease in the protein level of Ran in larvae administered bmnpv-miR-1 compared to control larvae (DEPC-H₂O administration). The band ratio was determined by densitometry and normalized against internal control α-tubulin. (C) Ran transcript analysis by RT-qPCR in BmN cells upon bmnpv-miR-1 transfection. For transfection, siRNA against GFP (siGFP) was used as a negative control. For RT-qPCR analysis, three independent experiments were carried out in three replicates, each with a set of 3 larvae, and the results were normalized against endogenous 18S rRNA. The data are presented as means ± SD (n = 3).