Fig 6.

bmnpv-miR-1-mediated repression of Ran hampers small-RNA transport in B. mori larvae and in BmN cells. (A) Northern blot analysis of B. mori miRNAs and lysine tRNA expression in larvae administered bmnpv-miR-1. Larvae administered DEPC-H₂O were used as a negative control. (B) bmnpv-miR-1 effects on host miRNAs, bmo-miR-1, bmo-let-7, and lysine tRNA were analyzed by Northern blotting. siGFP- and bmnpv-miR-1-transfected BmN cells are represented as siGFP and bmnpv-miR-1, respectively. The probes used are shown beneath each of the blots. The band ratio was determined by densitometry and normalized against endogenous control 5S rRNA.