Fig 7.

Inhibition of cellular miRNA, bmo-miR-8, results in greater BmNPV proliferation in B. mori larvae. (A) Transcript analysis of BmNPV ie-1 by RT-qPCR in BmNPV-infected larvae administered LNA-8 and scrambled LNA. (B) BmNPV loads in BmNPV-infected larvae administered LNA-8 and scrambled LNA were determined by qPCR of viral DNA using BmNPV ie-1-specific primers. For qPCR analysis, three independent experiments were carried out in three replicates each with a set of 3 larvae, and the results were normalized against endogenous 18S rRNA. The data are presented as means ± SD (n = 3). (C) Northern blots showing reduction in expression of bmo-miR-8 upon BmNPV infection in B. mori larvae. The band ratio was determined by densitometry and normalized against endogenous 5S rRNA.