Fig 8.

Blocking of bmnpv-miR-1 by LNA leads to a decrease in BmNPV accumulation in B. mori larvae. (A) RT-qPCR analysis shows an increase in Ran expression upon inhibition of bmnpv-miR-1 by specific LNA (LNA-1), while scrambled LNA has no effect compared to the respective control samples. (B) BmNPV titers, determined by qPCR using ie-1 of BmNPV, showed drastic reduction in the virus load when bmnpv-miR-1 was blocked by specific LNA (LNA-1), whereas no significant decrease in the virus load was observed in larvae administered scrambled LNA compared to control samples. Three independent experiments were carried out in three replicates each with a set of 3 larvae, and the results were normalized against the constitutively expressed 18S rRNA gene of B. mori. (C) The virus load was determined by scoring the OBs from hemolymph of BmNPV-infected larvae administered LNA. Three independent experiments were performed with a set of 3 larvae. The data are presented as means ± SD (n = 3).