Fig 4.

LEC permeability responses to ANDV infection. (A) LECs were plated on vitronectin-coated Transwell inserts and were ANDV, HTNV, or TULV infected at an MOI of 0.5 in triplicate or mock infected. Three days postinfection, FITC-dextran was added to media in the upper chamber in the presence or absence of VEGF-A (100 ng/ml), and 3 h later, FITC-dextran in the lower chamber was quantitated by fluorimetry as described previously (29). Results are expressed as fold increases in permeability to FITC-dextran over VEGF-A-treated mock-infected controls. (B) Permeability of ANDV-infected cells to FITC-dextran was determined as in panel A above after VEGF-A or VEGF-C (100 ng/ml) was added as indicated. (C) LECs were infected with ANDV, and at 3 days postinfection, VEGF-A or VEGF-C was added as indicated (100 ng/ml). Three hours later, the presence of ANDV-infected giant LECs was quantitated and presented as a percentage of mock-treated ANDV-infected LECs. Data represents results of four independent experiments (**, P < 0.01 versus control).