Fig 6.

vIL-8 binds to and attracts B cells. (A) Recombinant proteins used for binding and chemotaxis assay produced using the baculovirus expression system. (B) Binding assay of recombinant vIL-8 to B cells (left), CD4⁺ T cells (middle), or CD8⁺ T cells (right). Data are shown as histograms and are representative for three independent experiments. The percentage of vIL-8-positive cells is indicated. (C) Chemotaxis assay using vIL-8 as a chemoattractant for chicken blood PBMCs. Migrated cells were counted for the indicated time intervals by flow cytometry. Data are shown as a percentage of input cells normalized against background migration. Mean of 4 independent experiments (*, for count at 30 s [n = 12], P = 0.042 [vIL-8 versus Fc] and P = 0.089 [vIL-8 versus 9E3/CEF4]; for count at 120 s [n = 12], P = 0.011 [vIL-8 versus Fc] and P = 0.013 [vIL-8 versus 9E3/CEF4] [P values by Bonferroni correction]). (D) Analysis of cells that migrated in chemotaxis assays. Data are presented as the mean percentage of B cells or CD4⁺ or CD8⁺ T cells for each sample from three independent experiments (**, for B cells, P = 0.008; for CD4⁺ T cells, P > 0.05; for CD8⁺ T cells, P > 0.05 [P values by Bonferroni correction for vIL-8–huFc compared to either input or huFc]).